eclipse c1 immunofluorescence microscope  (Nikon)

Journal: Journal of Orthopaedic Translation

Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage

doi: 10.1016/j.jot.2025.101035

Figure Lengend Snippet: IChondrocyte-sEVs promoted chondrogenesis. a Representative image of safranin O and Alcian blue staining of ATDC5 cells after 14 days of chondrogenesis induction in a 24-well plate (n = 5). b Proliferation curves of ATDC5 cells were monitored from day 1 to day 4 (n = 5). c Representative images of histopathological and immunofluorescence staining of MSCs following 21 days of chondrogenesis induction (n = 4). Scale bars: black = 100 um, white = 20 μm d Quantitative analysis of the average fluorescence intensity of COLII, ACAN and SOX9 based on the results shown in ( c ) (n = 4). e Relative mRNA expression levels of anabolic genes in MSCs after 21 days of chondrogenesis induction (n = 6). f Western blotting analysis of COLII, ACAN and SOX9 in MSCs after 21 days of chondrogenesis induction (n = 4). Data are presented as mean ± SD. ∗P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ## P < 0.01 vs. control group.

Article Snippet: The slices were sealed and stored at −20 °C for immunofluorescence imaging (FV 3000, Olympus, Japan).

Techniques: Staining, Immunofluorescence, Fluorescence, Expressing, Western Blot, Control

Journal: Journal of Orthopaedic Translation

Article Title: Extracellular vesicles originating from induced pluripotent stem cell-derived chondrocytes facilitate the regeneration of osteoarthritic cartilage

doi: 10.1016/j.jot.2025.101035

Figure Lengend Snippet: IChondrocyte-sEVs inhibited fibrosis and calcification in MSC-derived chondrocyte-pellets, as well as ameliorated impairment in OA chondrocytes. a Representative immunofluorescence images of MSC-derived chondrocytes pellets (n = 4). Scale bar: 20 μm b Quantitative analysis of mean fluorescence intensity for COLX and COLI across experimental groups (n = 4). c Microstructural characterization of MSC-derived chondrocyte pellets with μCT and SEM (n = 4). Calcified regions are indicated by green fluorescence, with calcium salt deposition marked by red arrows. Scale bar: white = 500 μm, black = 20 μm d Quantitative assessment of bone volume and bone volume percentage in MSC-derived chondrocytes pellets (n = 4). e OA chondrocytes proliferation was assessed over a 4-day period (n = 3). f Representative images illustrating cellular morphology, Safranin O staining, and Alcian blue staining of chondrocytes (n = 3). Scale bar:200 um. g Relative mRNA expression levels of COL2A1 and ACAN genes in chondrocytes (n = 6). h Western blotting analysis of COLII and ACAN in chondrocytes (n = 4). Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, ### P < 0.001 vs. control group.

Article Snippet: The slices were sealed and stored at −20 °C for immunofluorescence imaging (FV 3000, Olympus, Japan).

Techniques: Derivative Assay, Immunofluorescence, Fluorescence, Staining, Expressing, Western Blot, Control